Hagen Matzeit studied voice and vocal pedagogy as a baritone in Berlin with Bernd Riedel. He began his singing career as a scholarship holder of the Komische Oper Berlin, where he has since performed in a variety of roles, first as a baritone, later also as a countertenor.

Contemporary music productions include: the role of Hans Scholl (Weiße Rose / White Rose) at the Théâtre de la Monnaie in Brussels, the title role in "Jakob Lenz" at the Teatro Colon in Buenos Aires, Prince Clearte in the world premiere of the opera "HYBRIS / Niobe" by Adreana Hölszky at the Schwetzingen SWR Festival, Majnun in the world premiere of "Leila and Majnun" by Samir Odeh-Tamimi at the Ruhrtriennale and the title role in "Jakub Flügelbunt"(world premiere) by Miroslav Srnka at the Semperoper Dresden. At the Festival of Aix-en-Provence he made his debut in the leading role of Aymar in the opera "Thanks to my Eyes" by Oscar Bianchi with the Ensemble Modern under the baton of Franck Ollu, directed by Joël Pommerat. This world premiere was broadcasted live in co-production with ARTE TV. Subsequently, Hagen Matzeit also made guest appearances in this production at renowned venues such as the Théâtre Royal de la Monnaie in Brussels, the Fondation Gulbenkian in Lisbon and others. In 2016/17 Hagen Matzeit sang the role of Dollfuß in the opera "Staatsoperette" at the Neue Oper in Vienna. The world premiere of "Das Rätsel der gestohlenen Stimme" brought him back to the Semperoper Dresden in 2017-18, this time in the role of Akustikus. A revival took place in 2018-19. In 2018-19 he also appeared as the Herald in Reimann's opera "Medea" at the Aalto-Theater Essen to great acclaim. In 2020 he could be heard at the Bregenz Festival as Orfeo in the world premiere of the opera "Impresario Dotcom" by Ľubica Čekovská (co-production with the Slovak National Theater in Bratislava). Plans for 2020-21 include the role of Jack Natters / Jago in the world premiere of Bernhard Lang's opera "Der Hetzer" at the Dortmund Theater, and the role of Prince Go-Go in Le Grand Macabre at the Prague State Opera. He will return to the Bregenz Festival in 2021 for the leading role of Poliphilo in the world premiere of Alexander Moosbrugger's opera "Wind".